description link name Arrays (including microarrays) are a set of probes immobilized on a surface. The probes can be oligonucleotides, cDNAs, antibodies and other molecules that recognize a target. Microarrays can be constructed by several methods including (but not limited to in situ oligo synthesis (e.g. Affymetrix), cDNA spotting, bead arrays (e.g. Illumina) and antibody spotting. The position and identity of probes are provided by the manufacturer. The probe identifiers and their target are referred to as annotation or translation of probe identifiers to bioinformatic identifiers. Microarrays can be used for gene expression (mRNA transcript quantification), genotyping, cytokine quantification, etc. Microarrays for gene expression fall into two general classes- single channel and dual channel. The channel refers to the wavelength scanned for fluorescent signals. Affymetrix microarrays are obligatory single channel. Many commercial and non-commercial microarray manufacturers use two channel. http://purl.obolibrary.org/obo/OBI_0400147 ; http://purl.obolibrary.org/obo/OBI_0001204 ; http://purl.obolibrary.org/obo/OBI_0001307 ; http://purl.obolibrary.org/obo/OBI_0400149 Array Cytometry Time Of Flight CyTOF (DVS Sciences) or Mass cytometry, or , is a variation of flow cytometry in which antibodies are labeled with heavy metal ion tags rather than fluorochromes. Readout is by time-of-flight mass spectrometry. http://en.wikipedia.org/wiki/Mass_cytometry CyTOF An assay in which a series of beads coated with antibodies specific for different analytes and marked with discrete fluorescent labels are used to simultaneously capture and quantitate soluble analytes using flow cytometric analysis. http://purl.obolibrary.org/obo/OBI_0000920 Cytometric Bead Array Enzyme-Linked ImmunoSorbant Assay. Quantification of a molecule (e.g cytokine) by an antibody immobilization strategy. http://purl.obolibrary.org/obo/OBI_0000661 ELISA Enzyme-linked ImmunoSPOT. A variant of ELISA with increased resolution that allows quantifying the number of cells in a population that release a molecule (e.g. cytokine). http://purl.obolibrary.org/obo/OBI_0600031 ELISPOT Fluorescence Activated Cell Sorting. http://purl.obolibrary.org/obo/OBI_0000916 Flow Cytometry Quantitate serum antibody to a specific antigen by blocking agglutination of cells. http://purl.obolibrary.org/obo/OBI_0000875 Hemagglutination Inhibition Human Leukocyte Antigen typing. http://purl.obolibrary.org/obo/OBI_0000435 HLA Typing Killer cell immunoglobulin-like receptors. http://purl.obolibrary.org/obo/OBI_0000435 KIR Typing Microsphere based multiplexing system. Microspheres are color coded and linked to a detector or capture reagent (e.g. antibody, oligonucleotides, peptides, or receptors). http://purl.obolibrary.org/obo/OBI_0000920 Luminex xMAP Measurement of how much antibody an organism has produced that recognizes a particular epitope, expressed as the inverse of the greatest dilution that still gives a positive result. http://purl.obolibrary.org/obo/VO_0000397 Neutralizing Antibody Titer Other reagent. http://immport.org/immport-open/public/home/documentation Other Polymerase Chain Reaction is a technique to amplify a DNA template. http://purl.obolibrary.org/obo/OBI_0000415 PCR Sequencing is used to discover new sequence variants and to genotype a sample for known variants. http://purl.obolibrary.org/obo/OBI_0600047 Sequencing Block a viral function. http://purl.obolibrary.org/obo/OBI_0000872 Virus Neutralization